{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325693/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325693"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The inhibition of miR-495-3p rescues Tsc1 knockdown-driven RNA expression changes","description":"Defective social behavior and cognitive functions are hallmarks of many neurodevelopmental disorders, most notably Autism Spectrum Disorders (ASD). We have identified the miR-379-410 cluster, the largest placental mammal-specific miRNA cluster, as a critical molecular brake on sociability. In this study, we utilized transient hippocampal knockdown (KD) of the ASD risk gene Tsc1, a key negative regulator of mTORC1, as a mouse model of autism-like behavior. Molecularly, Tsc1 KD leads to the upregulation of the miR-379-410 member, miR-495-3p. We found that the inhibition of this miRNA by a Locked Nucleic Acid(LNA)-modified antisense oligonucleotide was sufficient to prevent Tsc1 KD-induced hyposociability and memory impairments. To investigate the underlying transcriptomic changes, we assessed gene expression patterns in mouse hippocampi injected with either a control short hairpin, a Tsc1 short hairpin (shTsc1), or a combination of shTsc1 and a LNA against miR-495-3p. Our findings suggest that miR-495-3p is a key downstream effector of the Tsc1/mTORC1 pathway in the control of sociability, and that targeting miR-495-3p successfully rescues aberrant gene expression patterns back to physiological control levels.","dates":{"publication":"2026/09/01"},"accession":"GSE325693","cross_references":{"GSM":["GSM9610843","GSM9610833","GSM9610844","GSM9610841","GSM9610842","GSM9610847","GSM9610836","GSM9610837","GSM9610845","GSM9610834","GSM9610846","GSM9610835","GSM9610840","GSM9610838","GSM9610839"],"GPL":["34290"],"GSE":["325693"],"taxon":["Mus musculus"]}}