{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325822/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325822"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Bimodal Imaging-Guided Temporal Optimization of Combined Chemotherapy and DC Vaccine Immunotherapy","description":"Dendritic cell (DC) vaccines represent a clinically promising strategy for cancer treatment using a \"prime-boost\" approach. Achieving synergistic antitumor efficacy with chemotherapy requires precise temporal coordination, yet the timing of post-chemotherapy vaccination remains unstandardized, hindering its clinical translation. To address this, we designed a nanoprobe (Fe@O-DT(Cy7)) and applied bimodal imaging (FMI/MPI) to track DC vaccine migration and apoptosis after oxaliplatin treatment in a colorectal cancer model. We identified an optimal therapeutic window on day 7 post-chemotherapy, coinciding with peak immunogenic cell death (ICD). DC vaccination within this 7-d window ensured optimal lymph node homing, minimal apoptosis, and enhanced antitumor efficacy, characterized by immune cell infiltration and tertiary lymphoid structure (TLS) formation. Mechanistically, this window correlated with NF-κB pathway activation and CCL19/CCL21 upregulation. This study elucidates the role of the LTα1β2-LTβR axis in ICD-modulated DC vaccination and reveals the function of TLS in DC vaccine efficacy.","dates":{"publication":"2026/04/02"},"accession":"GSE325822","cross_references":{"GSM":["GSM9613958","GSM9613936","GSM9613937","GSM9613959","GSM9613938","GSM9613939","GSM9613954","GSM9613932","GSM9613955","GSM9613933","GSM9613934","GSM9613956","GSM9613957","GSM9613935","GSM9613972","GSM9613950","GSM9613951","GSM9613973","GSM9613974","GSM9613952","GSM9613930","GSM9613931","GSM9613953","GSM9613970","GSM9613971","GSM9613969","GSM9613947","GSM9613948","GSM9613949","GSM9613943","GSM9613965","GSM9613966","GSM9613944","GSM9613945","GSM9613967","GSM9613946","GSM9613968","GSM9613961","GSM9613940","GSM9613962","GSM9613963","GSM9613941","GSM9613964","GSM9613942","GSM9613960"],"GPL":["34290"],"GSE":["325822"],"taxon":["Mus musculus"]}}