GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325822/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325822GEOGSEfalseBimodal Imaging-Guided Temporal Optimization of Combined Chemotherapy and DC Vaccine ImmunotherapyDendritic cell (DC) vaccines represent a clinically promising strategy for cancer treatment using a "prime-boost" approach. Achieving synergistic antitumor efficacy with chemotherapy requires precise temporal coordination, yet the timing of post-chemotherapy vaccination remains unstandardized, hindering its clinical translation. To address this, we designed a nanoprobe (Fe@O-DT(Cy7)) and applied bimodal imaging (FMI/MPI) to track DC vaccine migration and apoptosis after oxaliplatin treatment in a colorectal cancer model. We identified an optimal therapeutic window on day 7 post-chemotherapy, coinciding with peak immunogenic cell death (ICD). DC vaccination within this 7-d window ensured optimal lymph node homing, minimal apoptosis, and enhanced antitumor efficacy, characterized by immune cell infiltration and tertiary lymphoid structure (TLS) formation. Mechanistically, this window correlated with NF-κB pathway activation and CCL19/CCL21 upregulation. This study elucidates the role of the LTα1β2-LTβR axis in ICD-modulated DC vaccination and reveals the function of TLS in DC vaccine efficacy.2026/04/02GSE325822GSM9613958GSM9613936GSM9613937GSM9613959GSM9613938GSM9613939GSM9613954GSM9613932GSM9613955GSM9613933GSM9613934GSM9613956GSM9613957GSM9613935GSM9613972GSM9613950GSM9613951GSM9613973GSM9613974GSM9613952GSM9613930GSM9613931GSM9613953GSM9613970GSM9613971GSM9613969GSM9613947GSM9613948GSM9613949GSM9613943GSM9613965GSM9613966GSM9613944GSM9613945GSM9613967GSM9613946GSM9613968GSM9613961GSM9613940GSM9613962GSM9613963GSM9613941GSM9613964GSM9613942GSM961396034290325822Mus musculus