{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325877/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Drosophila melanogaster"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325877"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Cell-type-specific circadian and light-responsive transcriptional dynamics in adult Drosophila neurons","description":"The Drosophila adult central brain contains 240 circadian neurons, of which there are more than 25 different neuron subtypes based on connectomic data. Recent single cell RNA-seq (scRNAseq) characterization of these neurons “around the clock” also indicate a similar number of circadian neuron subtypes, but other conclusions from these transcriptomic studies warranted verifying and extending with other approaches. To this end: 1) We used the Drosophila Genetic Reference Panel (DGRP) multiplexing strategy to eliminate 10X batch effects and profile time points together; 2) Large numbers of single nuclei were sequenced (snRNA-seq), which was enabled because the new method EL-INTACT purifies nuclei from frozen heads; 3) We assayed 12 time points under both light–dark (LD) and constant darkness (DD) conditions. These approaches showed dramatic time of day separation of many circadian neuron types and enhanced time-of-day gene expression analysis. The data indicate that most of this regulation is transcriptional and circadian. There were however a small number of light-dependent transcripts, including a few that correspond to mammalian immediate-early genes. They probably play a role in the light-regulation of gene expression and behavior in specific neurons, perhaps circadian entrainment or phase-shifting. The results taken together provide a more comprehensive picture of gene expression heterogeneity within adult Drosophila circadian neurons including how intrinsic clock mechanisms and light cues are integrated across circadian neuron subtypes.","dates":{"publication":"2026/08/21"},"accession":"GSE325877","cross_references":{"GSM":["GSM9615957","GSM9615956","GSM9615958","GSM9615955"],"GPL":["33093"],"GSE":["325877"],"taxon":["Drosophila melanogaster"],"PMID":["[42709819]"]}}