GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325877/primaryOK200TranscriptomicsDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325877GEOGSEfalseCell-type-specific circadian and light-responsive transcriptional dynamics in adult Drosophila neuronsThe Drosophila adult central brain contains 240 circadian neurons, of which there are more than 25 different neuron subtypes based on connectomic data. Recent single cell RNA-seq (scRNAseq) characterization of these neurons “around the clock” also indicate a similar number of circadian neuron subtypes, but other conclusions from these transcriptomic studies warranted verifying and extending with other approaches. To this end: 1) We used the Drosophila Genetic Reference Panel (DGRP) multiplexing strategy to eliminate 10X batch effects and profile time points together; 2) Large numbers of single nuclei were sequenced (snRNA-seq), which was enabled because the new method EL-INTACT purifies nuclei from frozen heads; 3) We assayed 12 time points under both light–dark (LD) and constant darkness (DD) conditions. These approaches showed dramatic time of day separation of many circadian neuron types and enhanced time-of-day gene expression analysis. The data indicate that most of this regulation is transcriptional and circadian. There were however a small number of light-dependent transcripts, including a few that correspond to mammalian immediate-early genes. They probably play a role in the light-regulation of gene expression and behavior in specific neurons, perhaps circadian entrainment or phase-shifting. The results taken together provide a more comprehensive picture of gene expression heterogeneity within adult Drosophila circadian neurons including how intrinsic clock mechanisms and light cues are integrated across circadian neuron subtypes.2026/08/21GSE325877GSM9615957GSM9615956GSM9615958GSM961595533093325877Drosophila melanogaster