{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325947/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325947"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Smc3-GFP flox/flox mouse embryonic stem cells with conditional knockout","description":"To investigate the functional consequences in the absence of Smc3 in mouse embryonic stem cells (mESCs), we treated the Smc3 GFP flox/flox mESCs with 4-OHT to deplete the Smc3 alleles, and then performed RNA-seq on two independent Smc3 flox/flox GFP ESC lines before and after Cre-mediated excision. RNA-seq analysis revealed dysregulation of key genes governing pluripotency and lineage commitment, highlighting the indispensable role of cohesin in preserving the transcriptional identity of embryonic stem cells.","dates":{"publication":"2026/09/10"},"accession":"GSE325947","cross_references":{"GSM":["GSM9617646","GSM9617644","GSM9617645","GSM9617643"],"GPL":["24247"],"GSE":["325947"],"taxon":["Mus musculus"]}}