GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325961/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325961GEOGSEfalseMolecular signatures of maladaptive plasticity in the amygdala in a rat model of chronic neuropathic painChronic pain, a complex multidimensional disorder, remains a major health care issue and a therapeutic challenge. Neuropathic pain is a chronic pain condition that results from damage or dysfunction in the nervous system. While mechanisms of neuropathic pain at the peripheral and spinal cord level have been extensively studied, pain mechanisms in the brain remain underexplored. The amygdala, a limbic brain region, has emerged as a critical brain area for the emotional-affective dimension of pain and pain modulation. Amygdala neuroplasticity has been associated with pain states, but exact molecular and cellular mechanisms underlying these states and the transition from acute to chronic pain are not well understood.We used an integrative approach that focuses on functional significance and cell type specificity of differentially expressed genes (DEGs) to nominate mechanistic targets for central regulation of chronic pain. Our integrative transcriptomic and bioinformatic analyses identified individual genes (e.g., Cxcl10, Cxcl12, Mbp, Plp1, Mag, Mog, Slc17a6, Gad1, Sst), molecular pathways (e.g., cytokine-mediated signaling pathway), biological processes (e.g., myelination, synaptic transmission), and specific cell types (e.g., oligodendrocytes, glutamatergic and GABA-ergic neurons) affected by chronic pain. Our results also provide evidence for the emerging concept of hemispheric lateralization of pain processing in the amygdala. Overall, our study proposes oligodendrocyte dysfunction in the amygdala, neuroimmune signaling in CeA, and glutamatergic neurotransmission in BLA as mechanistic determinants of and potential therapeutic targets for the management of chronic neuropathic pain.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 norvegicus