{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326233/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326233"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic effects of intermittent parathyroid hormone treatment and osteoprotegrin-Fc on Ebf3-lineage skeletal stem/progenitors","description":"We employed 10X Genomics single cell RNA-sequencing to study the bone anabolic effects of intermittent parathyroid hormone treatment (iPTH) and osteoprotegerin-Fc (OPG-Fc) on subsets of bone marrow Cxcl12-abundant reticular (CAR) cells. CAR cells, marked by the expression of the transcription factor Early B-cell Factor 3 (Ebf3), are a critical class of osteoblast progenitors participating in bone remodeling during adulthood. Using lineage tracing approach with Ebf3-CreERT2 crossed with Ai9 tdTomato reporter mice, we traced Ebf3-expressing CAR cells and their descendants following 10 days of vehicle or iPTH treatment. This study represents a single cell atlas of bone marrow Ebf3-lineage CAR cells following 10 days of iPTH treatment.","dates":{"publication":"2026/09/20"},"accession":"GSE326233","cross_references":{"GSM":["GSM9625633"],"GPL":["30172"],"GSE":["326233"],"taxon":["Mus musculus"]}}