GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326233/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326233GEOGSEfalseTranscriptomic effects of intermittent parathyroid hormone treatment and osteoprotegrin-Fc on Ebf3-lineage skeletal stem/progenitorsWe employed 10X Genomics single cell RNA-sequencing to study the bone anabolic effects of intermittent parathyroid hormone treatment (iPTH) and osteoprotegerin-Fc (OPG-Fc) on subsets of bone marrow Cxcl12-abundant reticular (CAR) cells. CAR cells, marked by the expression of the transcription factor Early B-cell Factor 3 (Ebf3), are a critical class of osteoblast progenitors participating in bone remodeling during adulthood. Using lineage tracing approach with Ebf3-CreERT2 crossed with Ai9 tdTomato reporter mice, we traced Ebf3-expressing CAR cells and their descendants following 10 days of vehicle or iPTH treatment. This study represents a single cell atlas of bone marrow Ebf3-lineage CAR cells following 10 days of iPTH treatment.2026/09/20GSE326233GSM962563330172326233Mus musculus