GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326397/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326397GEOGSEfalseA study for intratumoral heterogeneity of glioblastoma [scRNA-seq]Intratumoral heterogeneity (ITH) is a key driver of therapy resistance in glioblastoma (GBM). This study established a five-gene signature-based gITH classifier through multi-omics analysis, demonstrating robust prognostic predictive value. High-gITH tumors exhibited enhanced molecular complexity, with PDLIM4 identified as the central regulator showing strong correlations with stem-like properties and poor clinical outcomes. Functional validation confirmed that PDLIM4 knockdown suppressed ITH and tumor progression. Our work not only establishes a transcriptome-based quantification framework for GBM heterogeneity, but also reveals PDLIM4 as a promising therapeutic target, offering novel precision medicine strategies.2026/04/02GSE326397GSM9630417GSM963041524247326397Mus musculus