GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326505/primaryOK200TranscriptomicsSchizosaccharomyces pombeExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326505GEOGSEfalseDifferential gene expression drives cell-cycle-dependent transition from monopolar to bipolar growth in fission yeastIn S. pombe, cells transition from monopolar to bipolar growth in a cell cycle-dependent manner. To investigate this, we performed RNA-seq analysis of the cell-cycle mutant cdc10-129 to identify distinct gene expression patterns in G1 versus G2 phase. Our analysis identified upregulation of the pheromone- and stress-response pathways in G1/S-arrested cells and cell wall organization genes in G2 cells. Protein interaction networks identified a spk1-byr2-ste11 stress-response hub in G1/S. in agreement we find that cells spk1 are precociously bipolar. We propose that pheromone-response pathways block bipolarity until nutrient-rich conditions downregulate these pathways, enabling cell cycle progression and bipolar transition.2026/03/31GSE326505GSM9632896GSM9632885GSM9632897GSM9632886GSM9632898GSM9632887GSM9632888GSM9632899GSM9632900GSM9632889GSM9632901GSM9632890GSM9632891GSM9632892GSM9632893GSM9632894GSM9632895GSM963288428961326505Schizosaccharomyces pombe