{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326649/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Other"],"species":["Macaca mulatta"],"gds_type":[" Expression profiling by high throughput sequencing","Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326649"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Vaccination elicits HIV broadly neutralizing antibodies in primates","description":"The high antigenic diversity of HIV has been a major obstacle to development of a broadly protective vaccine. Nevertheless, protective HIV broadly neutralizing antibodies (bnAbs) exist and have been proposed as templates for vaccine development1-6. Germline-targeting is a conceptually radical vaccine design approach to elicit bnAbs, aiming to prime rare bnAb-precursor B cells possessing pre-determined human genetic and structural features shared with template bnAbs, and then guide B cell affinity maturation to potent bnAb evolution with heterologous boosters7-11. Although the approach has shown promise in clinical12-17 and pre-clinical18-34 studies, it faces many immunological challenges and, to date, has not succeeded in generating bnAbs in humans or nontransgenic animals. Here, we report an adjuvanted protein germline-targeting vaccine tested in outbred nonhuman primates that successfully generated bnAb-class memory B cells and sera capable of neutralizing diverse HIV clinical isolates. bnAb clonal lineages were generated in ≥50% of animals, achieving up to 46% neutralization breadth compared to the reference bnAb. Vaccine-induced bnAbs exhibited precise structural mimicry of human bnAb interactions with HIV envelope (Env), matching the germline-targeting predictions. Furthermore, serum bnAb activity developed in 44% of animals; in the most striking instance serum bnAb titers reached levels expected to confer protection against diverse HIV isolates. These results demonstrate proof of principle that germline-targeting vaccines can reproducibly elicit prespecified classes of bnAbs to prespecified epitopes under endogenous conditions, supporting further optimization of this approach for HIV vaccine development.","dates":{"publication":"2026/06/24"},"accession":"GSE326649","cross_references":{"GSM":["GSM9636339","GSM9636453","GSM9636332","GSM9636452","GSM9636331","GSM9636334","GSM9636455","GSM9636454","GSM9636333","GSM9636457","GSM9636336","GSM9636456","GSM9636335","GSM9636459","GSM9636338","GSM9636337","GSM9636458","GSM9636451","GSM9636330","GSM9636450","GSM9636343","GSM9636464","GSM9636342","GSM9636463","GSM9636345","GSM9636466","GSM9636465","GSM9636344","GSM9636468","GSM9636347","GSM9636346","GSM9636467","GSM9636349","GSM9636348","GSM9636469","GSM9636460","GSM9636462","GSM9636341","GSM9636340","GSM9636461","GSM9785375","GSM9785376","GSM9785377","GSM9636475","GSM9636354","GSM9636474","GSM9636353","GSM9636477","GSM9636356","GSM9636355","GSM9636476","GSM9636358","GSM9785378","GSM9785379","GSM9636478","GSM9636357","GSM9636359","GSM9636471","GSM9636350","GSM9636470","GSM9636352","GSM9636473","GSM9636472","GSM9636351","GSM9785385","GSM9636406","GSM9636405","GSM9785386","GSM9785387","GSM9636408","GSM9785388","GSM9636407","GSM9785381","GSM9785382","GSM9636409","GSM9785383","GSM9785384","GSM9636365","GSM9636364","GSM9636400","GSM9636367","GSM9636366","GSM9636402","GSM9636369","GSM9785389","GSM9636368","GSM9636401","GSM9636404","GSM9636403","GSM9636361","GSM9636360","GSM9636363","GSM9636362","GSM9785380","GSM9636417","GSM9636416","GSM9636419","GSM9636418","GSM9785392","GSM9636376","GSM9636375","GSM9636411","GSM9636378","GSM9636410","GSM9636377","GSM9636413","GSM9636379","GSM9636412","GSM9636415","GSM9636414","GSM9636370","GSM9636372","GSM9636371","GSM9636374","GSM9636373","GSM9785390","GSM9785391","GSM9636428","GSM9636427","GSM9636309","GSM9636308","GSM9636429","GSM9636420","GSM9636387","GSM9636386","GSM9636389","GSM9636422","GSM9636388","GSM9636421","GSM9636424","GSM9636423","GSM9636426","GSM9636425","GSM9636381","GSM9636380","GSM9636383","GSM9636382","GSM9636385","GSM9636384","GSM9636439","GSM9636318","GSM9636317","GSM9636438","GSM9636319","GSM9636398","GSM9636431","GSM9636310","GSM9636397","GSM9636430","GSM9636433","GSM9636312","GSM9636311","GSM9636432","GSM9636399","GSM9636314","GSM9636435","GSM9636434","GSM9636313","GSM9636437","GSM9636316","GSM9636436","GSM9636315","GSM9636390","GSM9636392","GSM9636391","GSM9636394","GSM9636393","GSM9636396","GSM9636395","GSM9636329","GSM9636449","GSM9636328","GSM9636321","GSM9636442","GSM9636320","GSM9636441","GSM9636323","GSM9636444","GSM9636443","GSM9636322","GSM9636446","GSM9636325","GSM9636445","GSM9636324","GSM9636448","GSM9636327","GSM9636326","GSM9636447","GSM9636440"],"GPL":["27943"],"GSE":["326649"],"taxon":["Macaca mulatta"]}}