{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326774/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326774"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"SNHG29 knockdown by CRISPRi and shRNA in M07e acute myeloid leukemia cells","description":"The long non-coding RNA SNHG29 has been identified as a candidate essential gene in acute myeloid leukemia (AML) through a genome-wide CRISPRi depletion screen. To characterize the transcriptomic consequences of SNHG29 loss, we performed RNA-seq in M07e AML cells following SNHG29 knockdown using two independent methods: CRISPRi (two sgRNAs targeting the SNHG29 promoter, with Luciferase-targeting sgRNA as control) and shRNA (two shRNAs targeting SNHG29, with scrambled shRNA as control). Three biological replicates were generated per condition. Differential expression analysis was performed using DESeq2.","dates":{"publication":"2026/06/30"},"accession":"GSE326774","cross_references":{"GSM":["GSM9639029","GSM9639028","GSM9639027","GSM9639026","GSM9639037","GSM9639032","GSM9639031","GSM9639030","GSM9639036","GSM9639025","GSM9639035","GSM9639024","GSM9639023","GSM9639034","GSM9639033"],"GPL":["24676"],"GSE":["326774"],"taxon":["Homo sapiens"]}}