GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326939/primaryOK200TranscriptomicsTrichomonas vaginalisExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326939GEOGSEfalseTranscriptomic signatures of Trichomonas vaginalis isolates that exhibit low, intermediate, and high resistance to metronidazole in vitroAs part of efforts to identify genes associated with Trichomonas vaginalis resistance to 5-nitroimidazole drugs, thirty cryopreserved T. vaginalis isolates were revived and grown using Diamonds TYM medium. Minimum lethal concentrations (MLCs) for metronidazole (MTZ), tinidazole (TDZ), and secnidazole (SEC) were determined using a drug susceptibility assay. Transcriptome profiling was performed for 15 MTZ-sensitive (MTZ-S, MLC < 50µg/mL) and 15 MTZ-resistant (MTZ-R, MLC ≥ 50µg/mL) isolates using next generation RNA-sequencing. Bioinformatics analyses identified differentially expressed genes (DEGs). Among MTZ-R isolates, 6 exhibited MLCs of 50µg/mL, 5 had MLCs between 100-200 µg/mL, and 4 had MLCs ≥ 400 µg/mL. The low-, intermediate-, and high-level MLC resistant isolates were compared to the MTZ-S isolates and transcriptomic profiling identified 154, 425, and 175 DEGs respectively. MTZ-resistant T. vaginalis isolates exhibited altered expression of virulence factor genes compared to sensitive isolates, with variations in gene expression observed across different MLC levels.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 vaginalis