GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327101/primaryOK200TranscriptomicsHomo sapiens Genome binding/occupancy profiling by high throughput sequencingExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327101GEOGSEfalseSingle-Cell Multimodal Atlas Reveals Regulatory Programs in the Human BrainDirectly measuring chromatin states alongside transcription is essential for understanding how cell-type-specific regulatory programs are established and maintained in the adult human brain. We present a large-scale single-cell multimodal atlas generated by jointly profiling transcriptome with active (H3K27ac) and repressive (H3K27me3) histone modifications across 18 brain regions. We profile >750,000 nuclei spanning 160 cell types and integrate these data with chromatin accessibility, DNA methylation, 3D genome architecture, and spatial transcriptome. This framework annotates >500,000 regulatory elements and resolves cell-type-specific chromatin states. We link enhancers to target genes, infer gene regulatory networks, and classify chromatin interactions, revealing neuron-enriched long-range Polycomb repression of developmental genes. Integrating these maps with GWAS data and sequence-based model prioritizes noncoding variants, effector genes, and vulnerable cell types for neuropsychiatric disorders. Finally, cross-species comparisons show conserved activation but more divergent repression. Together, this study provides a functional reference for interpreting noncoding variants, epigenetic memory, and brain organization.2026/06/26GSE327101GSM9660328GSM9660327GSM9660329GSM9648320GSM9648329GSM9660331GSM9648321GSM9648322GSM9660330GSM9648323GSM9660333GSM9648324GSM9660332GSM9660335GSM9648325GSM9660334GSM9648326GSM9660337GSM9648327GSM9660336GSM9648328GSM9660339GSM9660338GSM9648318GSM9648319GSM9660340GSM9648310GSM9648311GSM9660341GSM9648312GSM9648313GSM9648314GSM9648315GSM9648316GSM9648317GSM9660306GSM9660305GSM9660308GSM9660307GSM9648340GSM9660309GSM9648341GSM9648342GSM9648343GSM9648344GSM9660311GSM9648345GSM9648346GSM9660310GSM9648347GSM9660313GSM9660312GSM9648348GSM9648349GSM9660315GSM9660314GSM9660317GSM9660316GSM9660319GSM9660318GSM9648330GSM9648331GSM9648332GSM9660320GSM9648333GSM9648334GSM9660322GSM9648335GSM9660321GSM9648336GSM9660324GSM9648337GSM9660323GSM9660326GSM9648338GSM9660325GSM9648339GSM9660300GSM9660302GSM9660301GSM9660304GSM9660303GSM9648307GSM9648308GSM9648309GSM9648300GSM9648301GSM9648302GSM9648303GSM9648304GSM9648305GSM9648306GSM9660290GSM9660292GSM9660291GSM9660294GSM9660293GSM9660296GSM9660295GSM9660298GSM9660297GSM9660299GSM9648390GSM9648391GSM9648392GSM9648393GSM9648394GSM9648395GSM9648396GSM9648397GSM9648398GSM9648399GSM9660270GSM9660272GSM9660271GSM9660274GSM9660273GSM9660276GSM9660275GSM9660278GSM9660277GSM9660279GSM9648291GSM9648292GSM9648293GSM9648294GSM9648295GSM9648296GSM9648297GSM9648298GSM9648290GSM9660281GSM9660280GSM9660283GSM9660282GSM9660285GSM9660284GSM9648299GSM9660287GSM9660286GSM9660289GSM9660288GSM9648360GSM9648361GSM9648362GSM9648363GSM9648364GSM9660250GSM9660252GSM9660251GSM9660254GSM9648365GSM9648366GSM9660253GSM9660256GSM9648367GSM9660255GSM9648368GSM9648369GSM9660258GSM9660257GSM9660259GSM9648350GSM9648351GSM9648352GSM9648353GSM9660261GSM9660260GSM9660263GSM9660262GSM9648354GSM9660265GSM9648355GSM9660264GSM9648356GSM9660267GSM9648357GSM9660266GSM9648358GSM9660269GSM9660268GSM9648359GSM9648380GSM9648381GSM9648382GSM9648383GSM9648384GSM9648385GSM9648386GSM9660232GSM9648387GSM9648388GSM9660234GSM9648389GSM9660233GSM9660236GSM9660235GSM9660238GSM9660237GSM9660239GSM9648370GSM9648371GSM9648372GSM9648373GSM9648374GSM9648375GSM9660241GSM9660240GSM9648376GSM9660243GSM9648377GSM9660242GSM9660245GSM9648378GSM9648379GSM9660244GSM9660247GSM9660246GSM9660249GSM966024834281327101Homo sapiens[41676679]