GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327147/primaryOK200Othersynthetic construct Mus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327147GEOGSEfalseMERFISH data on seven mouse brain with Abeta pathologyMicroglia adopt various spatially resolved gene and functional profiles in amyloidosis mice, for example the relative enrichment of DAMs proximal to plaques. To spatially resolve these transcriptional changes and identify microglial populations responsive to TREM2 small molecule agonism, multiplexed error-robust fluorescence in situ hybridization (MERFISH) was conducted on sagittal brain sections from VG-3909–treated 5xFAD;hTREM2 mice, 12 hours post-oral dosing. Simultaneous immunostaining using an anti-amyloid antibody enabled spatial correlation of microglial transcriptomes with amyloid plaque localization. The custom MERFISH panel comprised 500 genes, included cell-type markers and microglia-focused genes.2026/04/24GSE327147GSM9649271GSM9649273GSM9649272GSM9649275GSM9649274GSM9649277GSM964927631217327147synthetic construct Mus musculus