{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327158/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327158"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A novel ocular fibrosis signature for AMD using transcriptomic profiling of the two-stage laser-induced subretinal fibrosis mouse model.","description":"Subretinal fibrosis is a major cause of irreversible vision loss in neovascular age-related macular degeneration (nAMD), yet conserved molecular signatures remain to be fully elucidated. Using RNA-seq on RPE/Choroid from a two-stage laser-induced subretinal fibrosis mouse model, we identified a novel core ocular fibrosis signature of 88 persistently upregulated genes across acute-to-chronic phases (days 3–10 post-injury). This signature, enriched in epithelial-mesenchymal transition, complement activation, and inflammatory pathways, showed choroid-specific expression with minimal retinal involvement. Cross-validation in independent chronic mouse data and multiple human AMD datasets (peripheral retina, surgically extracted CNV membranes, and macular RPE/choroid) confirmed progressive enrichment in advanced disease stages. Immunofluorescence in human fibrotic tissue validated key genes (Tenascin C, Tissue Inhibitor of Metalloproteinases-1, and Apelin receptor) with co-localization to myofibroblast-like cells and microglia. This persistent, cross-species-validated signature potentially highlights conserved fibrogenic drivers and offers a translational tool for biomarker development and targeted antifibrotic therapies in nAMD.","dates":{"publication":"2026/08/26"},"accession":"GSE327158","cross_references":{"GSM":["GSM9650132","GSM9650121","GSM9650120","GSM9650131","GSM9650130","GSM9650136","GSM9650125","GSM9650114","GSM9650135","GSM9650124","GSM9650113","GSM9650123","GSM9650134","GSM9650133","GSM9650122","GSM9650129","GSM9650118","GSM9650128","GSM9650117","GSM9650127","GSM9650116","GSM9650126","GSM9650115","GSM9650119"],"GPL":["30172"],"GSE":["327158"],"taxon":["Mus musculus"],"PMID":["[42615798]"]}}