GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327186/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327186GEOGSEfalseGNPs-pIL-4 reprograms macrophage polarization and activates the OSM/GSNOR/ENG axis to improve angiogenesis and tissue repair in murine ischemic limbsTherapeutic angiogenesis based on gene therapies is a potential peripheral artery disease (PAD) treatment approach. Here, we developed a graphene nanoparticle-based IL-4 plasmid delivery system (GNPs-pIL-4) to reprogram macrophage polarization and activate the OSM/GSNOR/ENG axis to improve angiogenesis and tissue repair in ischemic limbs. Single-cell RNA sequencing analysis revealed that GNPs-pIL-4 treatment significantly enhanced the number and strength of intercellular communications in ischemic tissues, with enrichment of pathways associated with endothelial sprouting and neovascularization.2026/07/03GSE327186GSM965068911002327186Mus musculus