{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327194/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327194"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Modelling B cell development with pluripotent stem cells","description":"The ability to generate functional B cells from human pluripotent stem cells (hPSCs) would open new opportunities to develop novel B cell-based therapies to treat a range of human diseases and disorders. Towards this goal, we established a MS-5 stromal based protocol that promotes the efficient development of B lineage cells from definitive hematopoietic progenitors generated from different hPSC lines. Flow cytometric and multi-omic scRNA-seq analyses revealed that B cell development from hPSCs transitions through the well-established pro- B, pre-B and naïve B cell stages, accurately recapitulating B lymphopoiesis in the human adult bone marrow.","dates":{"publication":"2026/08/21"},"accession":"GSE327194","cross_references":{"GSM":["GSM9651130","GSM9651126","GSM9651129","GSM9651128","GSM9651127"],"GPL":["24676"],"GSE":["327194"],"taxon":["Homo sapiens"],"PMID":["[42146710]"]}}