GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327194/primaryOK200TranscriptomicsHomo sapiens OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327194GEOGSEfalseModelling B cell development with pluripotent stem cellsThe ability to generate functional B cells from human pluripotent stem cells (hPSCs) would open new opportunities to develop novel B cell-based therapies to treat a range of human diseases and disorders. Towards this goal, we established a MS-5 stromal based protocol that promotes the efficient development of B lineage cells from definitive hematopoietic progenitors generated from different hPSC lines. Flow cytometric and multi-omic scRNA-seq analyses revealed that B cell development from hPSCs transitions through the well-established pro- B, pre-B and naïve B cell stages, accurately recapitulating B lymphopoiesis in the human adult bone marrow.2026/08/21GSE327194GSM9651130GSM9651126GSM9651129GSM9651128GSM965112724676327194Homo sapiens[42146710]