{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327262/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Saccharomyces cerevisiae"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327262"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Long telomeres induce a genome-wide transcriptional response in budding yeast","description":"Eukaryotes maintain telomere length within a defined range. While short telomeres are known to activate DNA damage responses and limit cell proliferation, long telomeres are associated with extended proliferative capacity, but their broader cellular consequences remain less well understood. In budding yeast Saccharomyces cerevisiae, long telomeres have been shown to influence gene expression at specific loci, but whether long telomeres affect transcription genome-wide has not been reported. Here, we analysed the transcriptomes of diploid yeast strains in a single lineage in which long telomeres were initially generated by a rif2delta mutation and subsequently inherited through successive rounds of mitosis and meiosis in the absence of this mutation. We show that strains with long telomeres exhibit a distinct gene expression profile, enriched in transcripts encoding membrane transporters. Both up- and down-regulated genes were distributed across the genome, arguing against a purely telomere-proximal effect on gene expression. Affected genes were enriched for Rap1 binding sites, consistent with a model in which long telomeres alter the availability of telomere-associated transcriptional regulators such as Rap1 and thereby affect gene expression at non-telomeric binding sites for these regulators. The magnitude of transcriptional changes was greatest in strains with the longest telomeres. Together, our findings demonstrate that long telomeres induce a genome-wide transcriptional response that accompanies inherited long telomeres across generations but diminishes as telomeres shorten. These types of transcriptional changes may also occur in other eukaryotes, including humans, where long telomeres can be inherited and are associated with disease.","dates":{"publication":"2026/04/13"},"accession":"GSE327262","cross_references":{"GSM":["GSM9652430","GSM9652431","GSM9652429","GSM9652436","GSM9652426","GSM9652437","GSM9652427","GSM9652428","GSM9652432","GSM9652433","GSM9652434","GSM9652435"],"GPL":["34739"],"GSE":["327262"],"taxon":["Saccharomyces cerevisiae"]}}