GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327277/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327277GEOGSEfalseBulk RNA-seq analysis of Delta-like 4 (Dll4) knockdown in LPS- or TNF-α-stimulated THP-1 cellsMonocyte-mediated inflammation, rather than T cell activation, is a major cause of chronic inflammation. Here, we investigated the contribution of delta-like 4 (Dll4)-Notch signaling in monocyte-mediated inflammation. To investigate the biological functions and molecular mechanisms of monocyte Dll4, bulk RNA sequencing (RNA-seq) was performed in THP-1 cells transfected with Dll4 siRNA or control siRNA for 24 hours in the presence of LPS or TNF-α stimulation. Four experimental groups were analyzed: control siRNA + LPS, Dll4 siRNA + LPS, control siRNA + TNF-α, and Dll4 siRNA + TNF-α. Each group included five biological replicates (n=5 per group). The RNA-seq analysis was designed as a discovery experiment powered to detect moderate-to-large expression differences.2026/07/02GSE327277GSM9652692GSM9652681GSM9652682GSM9652693GSM9652694GSM9652683GSM9652695GSM9652684GSM9652690GSM9652691GSM9652700GSM9652689GSM9652696GSM9652685GSM9652697GSM9652686GSM9652698GSM9652687GSM9652688GSM965269921697327277Homo sapiens[42283084]