{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327304/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327304"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic characterization of Wild type mouse Embryonic Stem Cells (mESCs), β-actin Knock out mESC and upon rescue with a nuclear localization signal β-actin","description":"Pluripotency and lineage commitment in embryonic stem cells depend on coordinated regulation of chromatin architecture and extracellular matrix (ECM) signaling. Here, we identify nuclear β-actin as a key regulator linking these processes in mouse embryonic stem cells. Loss of β-actin disrupts core pluripotency factors, including Oct4 and Sox2, and causes broad transcriptional changes, while nuclear re-expression rescues these defects. Chromatin accessibility analysis revealed reduced accessibility at regulatory regions of pluripotency genes, consistent with impaired chromatin remodeling. β-actin depletion also altered ECM-related gene expression, matrix properties, and cellular biomechanics, leading to impaired self-renewal, skewed lineage specification, and defective differentiation, particularly reduced neuronal potential and increased mesodermal-like fate bias. In vivo, β-actin loss restricted teratoma growth and compromised tri-lineage differentiation. Together, these results define nuclear β-actin as an important regulator of chromatin accessibility, ECM-dependent signaling, and stem cell fate.","dates":{"publication":"2026/04/13"},"accession":"GSE327304","cross_references":{"GSM":["GSM9653176","GSM9653154","GSM9653177","GSM9653155","GSM9653156","GSM9653178","GSM9653179","GSM9653157","GSM9653172","GSM9653173","GSM9653174","GSM9653152","GSM9653153","GSM9653175","GSM9653158","GSM9653159","GSM9653170","GSM9653171","GSM9653165","GSM9653166","GSM9653167","GSM9653168","GSM9653183","GSM9653161","GSM9653162","GSM9653184","GSM9653163","GSM9653164","GSM9653169","GSM9653180","GSM9653181","GSM9653182","GSM9653160"],"GPL":["24247"],"GSE":["327304"],"taxon":["Mus musculus"]}}