{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327332"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"JAX Study 13- RNA-seq of male KOLF2.2J hiPSC-derived endoderm and mesoderm homozygous null for ten different transcription factors","description":"This study aims to phenotype iPSC-derived early endoderm and mesoderm that contain homozygous null alleles for the following transcription factors: CITED1, HOXB8, GATA6, GSC, HES6, SP5, TBXT, LHX1, SOX17 and MSX1. Null alleles were generated using insertion of a premature termination codon with frameshift (PTC+1) in the case of CITED1, HOXB8, GATA6, SP5, TBXT, LHX1 and SOX17 or full protein coding region deletion (KO) for HES6 and MSX1. RNA-seq data was generated after 5 days of initiating differentiation from iPSCs. This analysis allowed the investigation of early embryonic development, cell fate mapping, and lineage analysis.","dates":{"publication":"2026/04/09"},"accession":"GSE327332","cross_references":{"GSM":["GSM9653750","GSM9653751","GSM9653734","GSM9653756","GSM9653757","GSM9653735","GSM9653758","GSM9653736","GSM9653759","GSM9653737","GSM9653752","GSM9653730","GSM9653731","GSM9653753","GSM9653754","GSM9653732","GSM9653755","GSM9653733","GSM9653749","GSM9653727","GSM9653728","GSM9653729","GSM9653760","GSM9653761","GSM9653762","GSM9653740","GSM9653745","GSM9653723","GSM9653746","GSM9653724","GSM9653725","GSM9653747","GSM9653748","GSM9653726","GSM9653763","GSM9653741","GSM9653742","GSM9653720","GSM9653764","GSM9653743","GSM9653721","GSM9653722","GSM9653744","GSM9653738","GSM9653739"],"GPL":["34281"],"GSE":["327332"],"taxon":["Homo sapiens"]}}