GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327332/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327332GEOGSEfalseJAX Study 13- RNA-seq of male KOLF2.2J hiPSC-derived endoderm and mesoderm homozygous null for ten different transcription factorsThis study aims to phenotype iPSC-derived early endoderm and mesoderm that contain homozygous null alleles for the following transcription factors: CITED1, HOXB8, GATA6, GSC, HES6, SP5, TBXT, LHX1, SOX17 and MSX1. Null alleles were generated using insertion of a premature termination codon with frameshift (PTC+1) in the case of CITED1, HOXB8, GATA6, SP5, TBXT, LHX1 and SOX17 or full protein coding region deletion (KO) for HES6 and MSX1. RNA-seq data was generated after 5 days of initiating differentiation from iPSCs. This analysis allowed the investigation of early embryonic development, cell fate mapping, and lineage analysis.2026/04/09GSE327332GSM9653750GSM9653751GSM9653734GSM9653756GSM9653757GSM9653735GSM9653758GSM9653736GSM9653759GSM9653737GSM9653752GSM9653730GSM9653731GSM9653753GSM9653754GSM9653732GSM9653755GSM9653733GSM9653749GSM9653727GSM9653728GSM9653729GSM9653760GSM9653761GSM9653762GSM9653740GSM9653745GSM9653723GSM9653746GSM9653724GSM9653725GSM9653747GSM9653748GSM9653726GSM9653763GSM9653741GSM9653742GSM9653720GSM9653764GSM9653743GSM9653721GSM9653722GSM9653744GSM9653738GSM965373934281327332Homo sapiens