GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327417/primaryOK200OtherHomo sapiens Expression profiling by high throughput sequencingOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327417GEOGSEfalseAtlas of human gut-associated lymphoid tissue reveals immunomodulatory interactions of B cellsGut-associated lymphoid tissue (GALT) is organized lymphoid tissue that responds chronically to antigens, including whole bacteria, sampled from the gut lumen. The ensuing immunoglobulin A (IgA) plasma cell response disseminates to regulate bacterial populations and to mediate intestinal immune homeostasis. GALT has roles in the development of the innate-like marginal zone B cell population and is also associated with a B cell–mediated contribution to ulcerative colitis (UC) severity and response to therapy. Applying integrated multiomics methodologies, we identified key spatially resolved interactions of B cell subsets including broad regulatory features of double negative 2 (DN2) B cells with potential to maintain homeostasis within microbe-rich mucosa. By contrast, GALT in UC is distorted in composition and spatial distribution of B cell subsets that have altered immunomodulatory potential compared to healthy GALT. Thus, we identify interactions of strategically located B cells as mediators of immunological equilibrium in human gut.2026/06/05GSE327417GSM9656510GSM9656512GSM9656511GSM9656507GSM9656506GSM9656509GSM9656508GSM9656514GSM9656513GSM9656505GSM9656515GSM965650416791327417Homo sapiens[42247482]