{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327462/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327462"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA binding protein YWHAZ mediates translation of specific mRNA and regulates cell Proliferation and apoptosis in diabetic foot ulcer","description":"YWHAZ was a significantly up-regulated and validated RBP in DFU by RT-qPCR and immunohistochemistry. Cellular experiments revealed that Si_YWHAZ facilitated proliferation and migration while inhibiting apoptosis, consistent with its upregulation in DFU. RNA-seq identified 1072 DEGs in Si_YWHAZ cells. Up-regulated genes were significantly enriched in cell proliferation-related processes and included AREG, FOSL1, HAS2 and IL7R, whereas down-regulated genes were associated with cell adhesion, such as LAMB3, SLAMF7, COL12A1 and ITGA5. IRIP-seq results demonstrated that YWHAZ interacts with plentiful of mRNAs and genomic location toward the CD and introns region. As well, ABLIFE algorithm indicated that YWHAZ bound to GC-rich motifs. Integrated iRIP-seq and RNA-seq analyses identified 57 DEGs that were selectively bound by YWHAZ, most of which were down-regulated. Notably, SREBF1, which was positively associated with type 2 diabetes risk. KEGG pathway analysis revealed SREBF1 is enriched in insulin resistance and insulin signaling pathway","dates":{"publication":"2026/07/29"},"accession":"GSE327462","cross_references":{"GSM":["GSM9657700","GSM9657705","GSM9657703","GSM9657704","GSM9657701","GSM9657702"],"GPL":["34284"],"GSE":["327462"],"taxon":["Homo sapiens"],"PMID":["[42180716]"]}}