{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327567/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327567"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Heat shock factor 5 is indispensable for pachynema progression and LINE1 silencing during spermatogenesis in mice [RNA-seq]","description":"Pachytene spermatocytes were isolated from adult wild-type and Hsf5 knockout mouse testes using STA-PUT velocity sedimentation. Single-cell suspensions were prepared by enzymatic digestion of seminiferous tubules with collagenase and trypsin. Cells were loaded onto a 4%–2%–0.5% BSA gradient and allowed to sediment for 1 hour and 45 minutes at 4°C. Fractions were collected, and pachytene spermatocytes were identified by SYCP3, γH2AX, and DAPI staining under microscopy. Enriched pachytene cells were pooled and processed for RNA-Seq.","dates":{"publication":"2026/04/14"},"accession":"GSE327567","cross_references":{"GSM":["GSM9661648","GSM9661647","GSM9661649","GSM9661646"],"GPL":["34290"],"GSE":["327567"],"taxon":["Mus musculus"]}}