{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327581/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327581"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell spatial transcriptomic profiling of 3xTg-AD mouse brains following fecal microbiota transplantation from stroke and healthy human donors","description":"Stroke-induced gut dysbiosis has been linked to accelerated neurodegeneration, but whether stroke-altered microbiota alone can initiate Alzheimer's disease (AD) pathology remains unclear. We performed fecal microbiota transplantation (FMT) from human stroke and healthy donors into pre-symptomatic 3xTg-AD mice and profiled brain-wide gene expression using the NanoString CosMx Spatial Molecular Imager. Single-cell spatial transcriptomics revealed extensive cell-type-specific transcriptional remodeling in Stroke-FMT mice, including upregulation of genes involved in amyloid processing, neuroinflammation, and lipid metabolism, alongside downregulation of autophagy and calcium-signaling pathways. Region-specific analyses of cortex and hippocampus demonstrated convergent upregulation of AD-relevant drivers including Apoe, App, and Grin2b. These transcriptomic changes were accompanied by elevated phospho-Tau, GFAP, and IBA1 immunoreactivity and disrupted gut-brain redox and energy metabolism.","dates":{"publication":"2026/04/13"},"accession":"GSE327581","cross_references":{"GSM":["GSM9661871","GSM9661873","GSM9661872","GSM9661875","GSM9661874"],"GPL":["36279"],"GSE":["327581"],"taxon":["Mus musculus"]}}