GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327779/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327779GEOGSEfalsemiR-151-5p Regulates Neural Stem Cell Fate by Targeting Aph1a to Modulate Notch Signaling GradientsTo investigate the role of miR-151-5p in regulating neural stem cell (NSC) fate during neocortical development, we performed RNA-seq profiling of NSCs isolated from the developing mouse cerebral cortex. Conditional knockout of miR-151-5p led to increased Sox2 expression and enhanced NSC proliferation. Mechanistically, Aph1a, a core subunit of the γ-secretase complex, was identified as a direct target of miR-151-5p. Overexpression of Aph1a phenocopied the knockout phenotype, promoting NSC proliferation via elevated NICD levels. Our results demonstrate that miR-151-5p biases NSC fate specification by targeting Aph1a to modulate Notch signaling, thereby fine-tuning the balance between NSC maintenance and differentiation. This study reveals a novel miR-151-5p/Aph1a/Notch signaling axis governing NSC fate, providing a critical post-transcriptional regulatory layer in mammalian neocortical development.2026/04/16GSE327779GSM9665808GSM9665807GSM9665806GSM9665805GSM9665804GSM96658039185327779Mus musculus