{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327794/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327794"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The periphery of nuclear speckles defines a spatially and temporally regulated compartment of long-lived intron-retained RNAs that resolves during mitosis","description":"RNA localization adds a fundamental layer to gene expression by determining when and where translation-ready mRNAs become available. Here, we identify a distinct subnuclear RNA niche at the nuclear speckle periphery that links intron retention to cell-cycle-timed RNA release. Using compartment-resolved transcriptional inhibition, sequence-based deep learning, and single-molecule and super-resolution RNA imaging in human pluripotent stem cells, we define a class of nuclear RNAs with long-lived retained introns (LL-IR-RNAs) that maintain stable intron retention for hours and are enriched in genome maintenance and mitosis regulators, including centromere and kinetochore assembly, DNA repair, and telomere maintenance. Long-lived retained introns are characterized by elevated GC content, predicted structural stability, and nuclear speckle-associated RBPs. In interphase, LL-IR-RNAs localize to a nuclear speckle-proximal niche conserved between cell types. During mitotic remodeling, they undergo coordinated, kinase-dependent splicing and populate the cytoplasm of early G1 daughter cells. These findings link cis-encoded intronic features, subnuclear organization and mitotic remodeling to temporal control of RNA fate.","dates":{"publication":"2026/06/24"},"accession":"GSE327794","cross_references":{"GSM":["GSM9666070","GSM9666063","GSM9666062","GSM9666061","GSM9666060","GSM9666067","GSM9666056","GSM9666066","GSM9666055","GSM9666065","GSM9666064","GSM9666059","GSM9666069","GSM9666058","GSM9666057","GSM9666068"],"GPL":["24676"],"GSE":["327794"],"taxon":["Homo sapiens"]}}