{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327802/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327802"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Histone H3K27 methylation states are sequentially catalyzed in cycling cells","description":"Polycomb domains form at silenced genes and are marked by histone H3 lysine 27 tri-methylation, but it is unclear how this methylation is maintained following DNA replication in proliferating cells. Here, we use CUT&Tag chromatin profiling to track the dynamics of H3K27 methylation in human cells. We find that tri-methylation in Polycomb domains is maintained by stepwise methylation after DNA replication. Outside of Polycomb domains, thousands of inactive genes gain histone H3K27 di-methylation hours after DNA replication. Acute treatment with small-molecule inhibitors of the Polycomb Repressive Complex 2 (PRC2) histone methyltransferase slows H3K27 methylation during S-phase of the cell cycle and increases acetylation of the residue. This effect is most pronounced at Polycomb domains replicating in early S-phase, suggesting that early replication and slow histone methylation confers plasticity to developmentally silenced genes. Together, our results explain how H3K27 modification patterns are faithfully duplicated in rapidly proliferating cells.","dates":{"publication":"2026/09/22"},"accession":"GSE327802","cross_references":{"GSM":["GSM9682909","GSM9682907","GSM9682908","GSM9682905","GSM9682906","GSM9682903","GSM9682904","GSM9682901","GSM9682902","GSM9682900","GSM9682920","GSM9666200","GSM9666201","GSM9666202","GSM9666203","GSM9682918","GSM9682919","GSM9666208","GSM9682916","GSM9682917","GSM9666209","GSM9682914","GSM9682915","GSM9666204","GSM9682912","GSM9666205","GSM9682913","GSM9682910","GSM9666206","GSM9666207","GSM9682911","GSM9683089","GSM9683087","GSM9666191","GSM9666190","GSM9683088","GSM9683085","GSM9683086","GSM9683083","GSM9666195","GSM9683084","GSM9666194","GSM9683081","GSM9666193","GSM9683082","GSM9666192","GSM9666199","GSM9683080","GSM9666198","GSM9666197","GSM9666196","GSM9683094","GSM9683095","GSM9683092","GSM9683093","GSM9683090","GSM9683091","GSM9682897","GSM9682898","GSM9682895","GSM9682896","GSM9682893","GSM9683069","GSM9682894","GSM9683067","GSM9682891","GSM9682892","GSM9683068","GSM9683065","GSM9682890","GSM9683066","GSM9683063","GSM9683064","GSM9683061","GSM9683062","GSM9683060","GSM9666179","GSM9666178","GSM9682888","GSM9682889","GSM9683078","GSM9683079","GSM9683076","GSM9666180","GSM9683077","GSM9683074","GSM9683075","GSM9666184","GSM9683072","GSM9683073","GSM9666183","GSM9683070","GSM9666182","GSM9666181","GSM9683071","GSM9666188","GSM9666187","GSM9666186","GSM9666185","GSM9666189","GSM9682899","GSM9682875","GSM9682996","GSM9682876","GSM9682997","GSM9682873","GSM9682994","GSM9683049","GSM9682995","GSM9682874","GSM9683047","GSM9682871","GSM9682992","GSM9682872","GSM9682993","GSM9683048","GSM9682990","GSM9683045","GSM9682870","GSM9682991","GSM9683046","GSM9683043","GSM9683044","GSM9683041","GSM9683042","GSM9683040","GSM9682868","GSM9682989","GSM9682869","GSM9682987","GSM9682866","GSM9682867","GSM9682988","GSM9682886","GSM9682887","GSM9682884","GSM9682885","GSM9683058","GSM9682882","GSM9683059","GSM9682883","GSM9683056","GSM9682880","GSM9682881","GSM9683057","GSM9683054","GSM9683055","GSM9683052","GSM9683053","GSM9683050","GSM9683051","GSM9682879","GSM9682998","GSM9682877","GSM9682878","GSM9682999","GSM9682853","GSM9682974","GSM9683029","GSM9682975","GSM9682854","GSM9683027","GSM9682851","GSM9682972","GSM9682852","GSM9682973","GSM9683028","GSM9682970","GSM9683025","GSM9682850","GSM9682971","GSM9683026","GSM9683023","GSM9683024","GSM9683021","GSM9683022","GSM9683020","GSM9682848","GSM9682969","GSM9682849","GSM9682967","GSM9682846","GSM9682847","GSM9682968","GSM9682844","GSM9682965","GSM9682845","GSM9682966","GSM9682864","GSM9682985","GSM9682865","GSM9682986","GSM9683038","GSM9682862","GSM9682983","GSM9682984","GSM9683039","GSM9682863","GSM9682981","GSM9683036","GSM9682860","GSM9682861","GSM9682982","GSM9683037","GSM9683034","GSM9682980","GSM9683035","GSM9683032","GSM9683033","GSM9683030","GSM9683031","GSM9682859","GSM9682978","GSM9682857","GSM9682858","GSM9682979","GSM9682855","GSM9682976","GSM9682856","GSM9682977","GSM9682952","GSM9683007","GSM9682953","GSM9683008","GSM9682950","GSM9683005","GSM9682951","GSM9683006","GSM9683003","GSM9683004","GSM9683001","GSM9683002","GSM9683000","GSM9666233","GSM9666234","GSM9666230","GSM9666231","GSM9666232","GSM9682949","GSM9682947","GSM9682948","GSM9682945","GSM9682946","GSM9682943","GSM9682944","GSM9683018","GSM9682842","GSM9682963","GSM9682964","GSM9683019","GSM9682843","GSM9682961","GSM9683016","GSM9682840","GSM9682841","GSM9682962","GSM9683017","GSM9683014","GSM9683015","GSM9682960","GSM9683012","GSM9683013","GSM9683010","GSM9683011","GSM9682839","GSM9682958","GSM9682837","GSM9682838","GSM9682959","GSM9682956","GSM9682836","GSM9682957","GSM9682954","GSM9683009","GSM9682955","GSM9682930","GSM9682931","GSM9666211","GSM9666212","GSM9666213","GSM9666214","GSM9682929","GSM9666210","GSM9682927","GSM9666219","GSM9682928","GSM9682925","GSM9682926","GSM9682923","GSM9666215","GSM9682924","GSM9666216","GSM9682921","GSM9666217","GSM9682922","GSM9666218","GSM9682941","GSM9682942","GSM9682940","GSM9666222","GSM9666223","GSM9666224","GSM9666225","GSM9666220","GSM9666221","GSM9682938","GSM9682939","GSM9682936","GSM9682937","GSM9682934","GSM9666226","GSM9666227","GSM9682935","GSM9682932","GSM9666228","GSM9682933","GSM9666229"],"GPL":["30173","34281"],"GSE":["327802"],"taxon":["Homo sapiens"]}}