GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327802/primaryOK200GenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327802GEOGSEfalseHistone H3K27 methylation states are sequentially catalyzed in cycling cellsPolycomb domains form at silenced genes and are marked by histone H3 lysine 27 tri-methylation, but it is unclear how this methylation is maintained following DNA replication in proliferating cells. Here, we use CUT&Tag chromatin profiling to track the dynamics of H3K27 methylation in human cells. We find that tri-methylation in Polycomb domains is maintained by stepwise methylation after DNA replication. Outside of Polycomb domains, thousands of inactive genes gain histone H3K27 di-methylation hours after DNA replication. Acute treatment with small-molecule inhibitors of the Polycomb Repressive Complex 2 (PRC2) histone methyltransferase slows H3K27 methylation during S-phase of the cell cycle and increases acetylation of the residue. This effect is most pronounced at Polycomb domains replicating in early S-phase, suggesting that early replication and slow histone methylation confers plasticity to developmentally silenced genes. Together, our results explain how H3K27 modification patterns are faithfully duplicated in rapidly proliferating cells.2026/09/22GSE327802GSM9682909GSM9682907GSM9682908GSM9682905GSM9682906GSM9682903GSM9682904GSM9682901GSM9682902GSM9682900GSM9682920GSM9666200GSM9666201GSM9666202GSM9666203GSM9682918GSM9682919GSM9666208GSM9682916GSM9682917GSM9666209GSM9682914GSM9682915GSM9666204GSM9682912GSM9666205GSM9682913GSM9682910GSM9666206GSM9666207GSM9682911GSM9683089GSM9683087GSM9666191GSM9666190GSM9683088GSM9683085GSM9683086GSM9683083GSM9666195GSM9683084GSM9666194GSM9683081GSM9666193GSM9683082GSM9666192GSM9666199GSM9683080GSM9666198GSM9666197GSM9666196GSM9683094GSM9683095GSM9683092GSM9683093GSM9683090GSM9683091GSM9682897GSM9682898GSM9682895GSM9682896GSM9682893GSM9683069GSM9682894GSM9683067GSM9682891GSM9682892GSM9683068GSM9683065GSM9682890GSM9683066GSM9683063GSM9683064GSM9683061GSM9683062GSM9683060GSM9666179GSM9666178GSM9682888GSM9682889GSM9683078GSM9683079GSM9683076GSM9666180GSM9683077GSM9683074GSM9683075GSM9666184GSM9683072GSM9683073GSM9666183GSM9683070GSM9666182GSM9666181GSM9683071GSM9666188GSM9666187GSM9666186GSM9666185GSM9666189GSM9682899GSM9682875GSM9682996GSM9682876GSM9682997GSM9682873GSM9682994GSM9683049GSM9682995GSM9682874GSM9683047GSM9682871GSM9682992GSM9682872GSM9682993GSM9683048GSM9682990GSM9683045GSM9682870GSM9682991GSM9683046GSM9683043GSM9683044GSM9683041GSM9683042GSM9683040GSM9682868GSM9682989GSM9682869GSM9682987GSM9682866GSM9682867GSM9682988GSM9682886GSM9682887GSM9682884GSM9682885GSM9683058GSM9682882GSM9683059GSM9682883GSM9683056GSM9682880GSM9682881GSM9683057GSM9683054GSM9683055GSM9683052GSM9683053GSM9683050GSM9683051GSM9682879GSM9682998GSM9682877GSM9682878GSM9682999GSM9682853GSM9682974GSM9683029GSM9682975GSM9682854GSM9683027GSM9682851GSM9682972GSM9682852GSM9682973GSM9683028GSM9682970GSM9683025GSM9682850GSM9682971GSM9683026GSM9683023GSM9683024GSM9683021GSM9683022GSM9683020GSM9682848GSM9682969GSM9682849GSM9682967GSM9682846GSM9682847GSM9682968GSM9682844GSM9682965GSM9682845GSM9682966GSM9682864GSM9682985GSM9682865GSM9682986GSM9683038GSM9682862GSM9682983GSM9682984GSM9683039GSM9682863GSM9682981GSM9683036GSM9682860GSM9682861GSM9682982GSM9683037GSM9683034GSM9682980GSM9683035GSM9683032GSM9683033GSM9683030GSM9683031GSM9682859GSM9682978GSM9682857GSM9682858GSM9682979GSM9682855GSM9682976GSM9682856GSM9682977GSM9682952GSM9683007GSM9682953GSM9683008GSM9682950GSM9683005GSM9682951GSM9683006GSM9683003GSM9683004GSM9683001GSM9683002GSM9683000GSM9666233GSM9666234GSM9666230GSM9666231GSM9666232GSM9682949GSM9682947GSM9682948GSM9682945GSM9682946GSM9682943GSM9682944GSM9683018GSM9682842GSM9682963GSM9682964GSM9683019GSM9682843GSM9682961GSM9683016GSM9682840GSM9682841GSM9682962GSM9683017GSM9683014GSM9683015GSM9682960GSM9683012GSM9683013GSM9683010GSM9683011GSM9682839GSM9682958GSM9682837GSM9682838GSM9682959GSM9682956GSM9682836GSM9682957GSM9682954GSM9683009GSM9682955GSM9682930GSM9682931GSM9666211GSM9666212GSM9666213GSM9666214GSM9682929GSM9666210GSM9682927GSM9666219GSM9682928GSM9682925GSM9682926GSM9682923GSM9666215GSM9682924GSM9666216GSM9682921GSM9666217GSM9682922GSM9666218GSM9682941GSM9682942GSM9682940GSM9666222GSM9666223GSM9666224GSM9666225GSM9666220GSM9666221GSM9682938GSM9682939GSM9682936GSM9682937GSM9682934GSM9666226GSM9666227GSM9682935GSM9682932GSM9666228GSM9682933GSM96662293017334281327802Homo sapiens