{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327833/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Bos taurus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327833"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"An engineered bovine mesenchymal stem cell platform enables scalable cultivated meat production","description":"Cultivated meat production requires cell platforms that combine long-term proliferative stability with efficient differentiation and compatibility with scalable manufacturing workflows. Mesenchymal stem cells (MSCs) are attractive in this context because of their adipogenic potential and relative ease of isolation and expansion, but primary MSCs undergo replicative senescence and typically show limited myogenic competence. Here, we developed an engineered bovine Wharton’s jelly derived MSC platform designed to address these limitations. We compared two immortalization strategies, hTERT and SV40 large T antigen, and found that hTERT-mediated immortalization maintained MSC marker expression and robust neutral lipid accumulation consistent with adipogenic induction, whereas SV40 immortalization was associated with marked genomic instability. However, although immortalized MSCs retained robust adipogenesis, myogenic differentiation remained inefficient. To overcome this bottleneck, we introduced an inducible MYOD program into WJ-hTERT cells. Upon doxycycline induction and exposure to a reduced myogenic differentiation cocktail, the engineered cells underwent rapid and efficient myogenic differentiation, with extensive multinucleated myotube formation, actin alignment, and induction of canonical myogenic markers. We further demonstrate that these cells are compatible with long-term 3D spheroid culture, enabling repeated weekly expansion cycles over more than 60 days, and can subsequently be transferred onto edible cellulose scaffolds for lineage-specific differentiation. On scaffolds, the cells supported both adipogenic remodeling and organized myogenic differentiation. In addition, pre-loading scaffolds with myogenic factors reduced the need for repeated growth factor supplementation during maturation, suggesting a route toward lower-cost differentiation workflows. Together, these results establish proof-of-concept modular bovine MSC-based cultivated meat platform in which controlled immortalization, inducible myogenic programming, suspension-compatible expansion, and scaffold-guided tissue formation are each validated and interoperable at the module level.","dates":{"publication":"2026/08/10"},"accession":"GSE327833","cross_references":{"GSM":["GSM9666871","GSM9666882","GSM9666883","GSM9666872","GSM9666884","GSM9666873","GSM9666874","GSM9666885","GSM9666880","GSM9666881","GSM9666870","GSM9666868","GSM9666879","GSM9666869","GSM9666875","GSM9666886","GSM9666887","GSM9666865","GSM9666876","GSM9666877","GSM9666866","GSM9666878","GSM9666867"],"GPL":["26012"],"GSE":["327833"],"taxon":["Bos taurus"]}}