{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328003/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328003"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Honokiol-mediated reversal of cisplatin-induced transcriptomic changes in outer hair cells","description":"Cisplatin-induced hearing loss (CIHL) affects half a million cancer patients annually, but treatment strategies are limited. We have previously shown that honokiol upregulates mitochondrial sirtuin 3 (SIRT3) and protects against CIHL. In the present study, we further investigated the mechanisms of honokiol and the role of Sirt3 in protection against CIHL by using a Sirt3 knockout (Sirt3−/−) mouse model and by performing RNA-seq on outer hair cells. Our results showed that honokiol ameliorated cisplatin-induced cochlear functional deterioration in both wild-type and Sirt3−/− mice. RNA-seq analysis showed that cisplatin treatment resulted in the downregulation of mitochondrial-related transcriptome and important antioxidant genes, such as isocitrate dehydrogenase 2 (Idh2). Honokiol pre-treatment reversed this pattern by upregulating genes suppressed by cisplatin. These results highlight the clinical potential of honokiol as a protective agent during chemotherapy, while suggesting that Idh2, a substrate of Sirt3, is a promising therapeutic target.","dates":{"publication":"2026/08/19"},"accession":"GSE328003","cross_references":{"GSM":["GSM9670209","GSM9670208","GSM9670229","GSM9670228","GSM9670227","GSM9670237","GSM9670215","GSM9670214","GSM9670236","GSM9670235","GSM9670213","GSM9670234","GSM9670212","GSM9670211","GSM9670233","GSM9670232","GSM9670210","GSM9670231","GSM9670230","GSM9670219","GSM9670218","GSM9670239","GSM9670217","GSM9670216","GSM9670238","GSM9670226","GSM9670225","GSM9670224","GSM9670223","GSM9670222","GSM9670221","GSM9670220"],"GPL":["34290"],"GSE":["328003"],"taxon":["Mus musculus"]}}