{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328010/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328010"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing of naïve, activated and HIV-1 infected CD4+ T cells","description":"CD4+ T cells play a major role in immune function regulation. Naive CD4+ T cells undergo major transcriptional changes upon activation through simultaneous engagement of the T-cell receptor and co-stimulatory molecules (anti-CD3/anti-CD28). Activated CD4+ T cells are the preferential target of HIV-1. Both T cell activation and HIV-1 infection modulate the expression and splicing of multiple genes. This study characterizes the transcriptome of naive, activated, and activated-HIV-1 infected CD4+ T cells from 3 healthy donors using bulk RNA-Seq, with validation of gene expression and alternative splicing by RT-qPCR and RT-PCR respectively.","dates":{"publication":"2026/04/14"},"accession":"GSE328010","cross_references":{"GSM":["GSM9670313","GSM9670312","GSM9670311","GSM9670310","GSM9670309","GSM9670308","GSM9670307","GSM9670306","GSM9670305"],"GPL":["16791"],"GSE":["328010"],"taxon":["Homo sapiens"]}}