GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328010/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328010GEOGSEfalseRNA sequencing of naïve, activated and HIV-1 infected CD4+ T cellsCD4+ T cells play a major role in immune function regulation. Naive CD4+ T cells undergo major transcriptional changes upon activation through simultaneous engagement of the T-cell receptor and co-stimulatory molecules (anti-CD3/anti-CD28). Activated CD4+ T cells are the preferential target of HIV-1. Both T cell activation and HIV-1 infection modulate the expression and splicing of multiple genes. This study characterizes the transcriptome of naive, activated, and activated-HIV-1 infected CD4+ T cells from 3 healthy donors using bulk RNA-Seq, with validation of gene expression and alternative splicing by RT-qPCR and RT-PCR respectively.2026/04/14GSE328010GSM9670313GSM9670312GSM9670311GSM9670310GSM9670309GSM9670308GSM9670307GSM9670306GSM967030516791328010Homo sapiens