{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328042/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328042"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq profiling of iPSC-derived microglia (iMicroglia) with wild-type or GPNMB knockout genotype","description":"Glycoprotein nonmetastatic melanoma B (GPNMB) is critical to cellular uptake of pathological forms of alpha-synuclein (aSyn), the hallmark disease protein in Parkinson's Disease (PD). Here, we demonstrate that the non-membrane-anchored, extracellular domain of GPNMB can function in a non-cell-autonomous manner. In human brain, GPNMB is widely expressed in neurons and microglia. In induced pluripotent stem cell-derived microglia (iMicroglia), GPNMB expression and secretion increase with exposure to apoptotic neurons. In the aSyn fibril-seeded model of PD, iMicroglia-derived GPNMB enhances neuronal aSyn uptake and development of aSyn pathology, including in GPNMB knockout neurons. This dataset contains bulk RNA-seq data from iPSC-derived microglia (iMicroglia) differentiated using the Brownjohn protocol from isogenic GPNMB wild-type (WT) and GPNMB knockout (KO) iPSC lines (3 biological replicates each), treated with PBS as vehicle control.","dates":{"publication":"2026/05/06"},"accession":"GSE328042","cross_references":{"GSM":["GSM9670909","GSM9670914","GSM9670913","GSM9670912","GSM9670911","GSM9670910"],"GPL":["30173"],"GSE":["328042"],"taxon":["Homo sapiens"]}}