GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328168/primary200OKOther Melampsora allii-populinaMelampsora laricis-populinaOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328168GEOGSEfalseHaplotype-phased assemblies of the two poplar rust fungi species: Melampsora larici-populina and Melampsora allii-populinaDikaryotic rust fungi maintain two distinct haploid nuclei for most of their life cycle, making their large, repeat-rich genomes difficult to assemble and phase. Here we present haplotype-phased, near chromosome-scale genome assemblies for the poplar rust pathogens Melampsora larici-populina 98AG31 and Melampsora allii-populina 12AY07, generated using PacBio HiFi sequencing and Hi-C-guided scaffolding. For each species, we resolve 18 chromosomes per haplotype, providing the first chromosome-level representations of poplar rust fungal species. M. larici-populina diploid assembly spans ~203 Mb, while M. allii-populina reaches ~416 Mb, with high completeness and strong collinearity between haplotypes.2026/06/24GSE328168GSM9674370GSM96743693682536824328168 Melampsora allii-populinaMelampsora laricis-populina