{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328211/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328211"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"eIF3 interactions with miRNAs involved in translational control of early T cell activation","description":"Early T cell activation induces extensive remodeling of the cellular transcriptome and proteome. We previously showed using a transcriptome-wide crosslinking approach that human translation initiation factor eIF3 directly interacts with a select set of immune-related mRNAs shortly after T cell activation. We also found that eIF3 binding to the 3’-untranslated regions (3’-UTRs) of the TCRA and TCRB mRNAs encoding the T cell receptor alpha and beta subunits dynamically regulates a burst in their translation. MicroRNAs (miRNAs) add an additional layer of regulation by fine-tuning both mRNA and protein expression. Although miRNA expression is dynamically regulated during T cell activation, how miRNAs interact with core regulatory pathways in primary T cells remains poorly understood. Here, we reexamined the eIF3-RNA crosslinking experiments in Jurkat cells and probed eIF3 function to investigate miRNA-mediated translational regulation during T cell activation. We found that human eIF3 interacts with multiple mature miRNAs in activated Jurkat cells, including members of the miR-17-92 cluster. These interactions also occur in primary T cells, as shown by RNA immunoprecipitation followed by qPCR (RIP-qPCR). Knocking out the miR-17-92 cluster led to a delay in ILR2A (CD25) cell surface expression in Jurkat cells and reduced activation-associated cell size increase in primary T cells during early T cell activation. In parallel, we used mass spectrometry analysis of activated primary T cells to identify eIF3-interacting factors, and surprisingly found no evidence of Argonaute binding. Together, these findings provide evidence that eIF3-miRNA interactions may play an unappreciated role in translational control during early T cell activation.","dates":{"publication":"2026/06/10"},"accession":"GSE328211","cross_references":{"GSM":["GSM9675063","GSM9675052","GSM9675062","GSM9675051","GSM9675065","GSM9675054","GSM9675064","GSM9675053","GSM9675056","GSM9675055","GSM9675058","GSM9675057","GSM9675049","GSM9675059","GSM9675048","GSM9675061","GSM9675050","GSM9675060"],"GPL":["24676"],"GSE":["328211"],"taxon":["Homo sapiens"]}}