GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328232/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328232GEOGSEfalseTranscriptomic Profiling Reveals the Impaired Oocyte Maturation and Dysregulated Molecular Transition from GV to MII Stage in a DHEA-induced PCOS Mouse ModelPolycystic ovary syndrome (PCOS) is a common endocrine disorder associated with impaired oocyte maturation, yet the transcriptomic mechanisms underlying oocyte developmental failure remain poorly understood. Here, we performed bulk RNA sequencing (Smart-seq) of mouse oocytes at two maturation stages — germinal vesicle (GV) and metaphase II (MII) — collected from normal control mice and a PCOS mouse model. A total of 14 samples across four groups (GV_Control, GV_PCOS, MII_Control, MII_PCOS) were profiled. Differential expression analysis revealed widespread transcriptomic dysregulation in PCOS oocytes, including large-scale failure of maternal mRNA clearance during maturation, impaired activation of RNA-binding proteins (RBPs) critical for mRNA decay, and aberrant suppression of oocyte identity transcription factors (Figla, Nobox). Trajectory analysis identified four categories of gene expression patterns disrupted in PCOS: impaired activation, aberrant suppression, failed clearance, and aberrant gain. These findings provide a comprehensive transcriptomic landscape of PCOS-associated oocyte maturation failure and identify Lsm14b as a key RBP whose activation is specifically impaired in PCOS oocytes.2026/08/19GSE328232GSM9675520GSM9675522GSM9675521GSM9675513GSM9675524GSM9675523GSM9675526GSM9675515GSM9675525GSM9675514GSM9675517GSM9675516GSM9675519GSM967551824247328232Mus musculus[42597781]