GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328254/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328254GEOGSEfalseCollagen-heparan sulfate microcarriers support mesenchymal stem cell culture in microgravity and streamline post-flight recoveryThe objective of this study was to validate a scalable, integrated workflow for Mesenchymal Stem Cell (MSC) culture in Low Earth Orbit (LEO) using pre-seeded, surface-engineered microcarriers (uCs). Cell thaw and expansion were conducted at ambient temperature (22 °C) in serum-free and DMSO-free media. Consequently, the primary objective of the miRNA sequencing was to verify the presence and stability of bioactive cargo within the extracellular vesicle (EV)-enriched fraction. This genomic analysis serves as a critical quality metric to ensure that the "pre-seed-freeze-thaw-and-expand" logistical framework preserves the integrity of the MSC secretome during orbital transit and post-flight. Small RNA sequencing was performed on EV-enriched fractions isolated from frozen conditioned media processed post-flight to assess the stability of bioactive miRNA signatures across different surface chemistries (engineered HS/COL vs. commercial COL uCs) and gravitational environments. MSCs in suspension (lacking microcarriers) served as the matrix-attachment control.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 sapiens[42624844]