{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328275/","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328275/suppl/GSE328275_processed_data.tar.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328275"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing of CD45+ immune cells across primary tumor, sentinel tumor-draining lymph node, and axillary lymph node in treatment-naive triple-negative breast cancer","description":"Regional lymph node metastasis critically influences distant metastatic progression, anti-tumor immunity, and patient prognosis in triple-negative breast cancer (TNBC). In this study, fresh surgically resected specimens from treatment-naive TNBC patients were collected and CD45+ immune cells were isolated for single-cell RNA sequencing to characterize immune remodeling across matched primary tumors, sentinel tumor-draining lymph nodes (S-TDLNs), and secondary axillary lymph nodes (ALNs). The overall project integrated scRNA-seq with imaging mass cytometry, Visium and Xenium spatial transcriptomics, and multiplex immunofluorescence. This GEO submission contains processed scRNA-seq expression matrices and associated metadata only. Raw sequencing data have been deposited previously in in the Genome Sequence Archive (GSA) for human data, and the GSA ID is HRA010347.","dates":{"publication":"2026/06/27"},"accession":"GSE328275","cross_references":{"GSM":["GSM9677561","GSM9677540","GSM9677560","GSM9677543","GSM9677544","GSM9677541","GSM9677542","GSM9677547","GSM9677548","GSM9677545","GSM9677546","GSM9677549","GSM9677550","GSM9677551","GSM9677554","GSM9677555","GSM9677552","GSM9677553","GSM9677536","GSM9677558","GSM9677537","GSM9677559","GSM9677556","GSM9677534","GSM9677557","GSM9677535","GSM9677538","GSM9677539"],"GPL":["34284"],"GSE":["328275"],"taxon":["Homo sapiens"]}}