GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328275/ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328275/suppl/GSE328275_processed_data.tar.gzprimaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328275GEOGSEfalseSingle-cell RNA sequencing of CD45+ immune cells across primary tumor, sentinel tumor-draining lymph node, and axillary lymph node in treatment-naive triple-negative breast cancerRegional lymph node metastasis critically influences distant metastatic progression, anti-tumor immunity, and patient prognosis in triple-negative breast cancer (TNBC). In this study, fresh surgically resected specimens from treatment-naive TNBC patients were collected and CD45+ immune cells were isolated for single-cell RNA sequencing to characterize immune remodeling across matched primary tumors, sentinel tumor-draining lymph nodes (S-TDLNs), and secondary axillary lymph nodes (ALNs). The overall project integrated scRNA-seq with imaging mass cytometry, Visium and Xenium spatial transcriptomics, and multiplex immunofluorescence. This GEO submission contains processed scRNA-seq expression matrices and associated metadata only. Raw sequencing data have been deposited previously in in the Genome Sequence Archive (GSA) for human data, and the GSA ID is HRA010347.2026/06/27GSE328275GSM9677561GSM9677540GSM9677560GSM9677543GSM9677544GSM9677541GSM9677542GSM9677547GSM9677548GSM9677545GSM9677546GSM9677549GSM9677550GSM9677551GSM9677554GSM9677555GSM9677552GSM9677553GSM9677536GSM9677558GSM9677537GSM9677559GSM9677556GSM9677534GSM9677557GSM9677535GSM9677538GSM967753934284328275Homo sapiens