GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328326/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328326GEOGSEfalseGene expression of pachytene-stage spermatocytes during male meiosis in Mus musculus [RNA-Seq]Inhibition of pre‑rRNA transcription by BMH21 (12 h) in ex vivo mouse testicular single‑cell suspensions, followed by FACS isolation of pachytene spermatocytes (Hoechst 33342) and RNA‑seq. This dataset is designed to assess whether perturbing pre‑rRNA accumulation within the XY body affects the transcriptional landscape of pachytene spermatocytes, particularly regarding meiotic sex chromosome inactivation (MSCI). Comparison of BMH21‑treated vs control cells identifies differentially expressed autosomal and sex‑linked genes, providing insights into the role of nucleolar activity in sex chromosome regulation during male meiosis.2026/05/28GSE328326GSM9679223GSM9679222GSM9679225GSM967922424247328326Mus musculus