{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328367/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"," Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328367"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Multiome-based identification of HSC subtype markers shows that ageing, but not inflammatory stress, increases HSC platelet bias","description":"Individual haematopoietic stem cells (HSCs) display heterogeneous capacities to reconstitute the major blood lineages, including platelets, erythrocytes, myeloid cells, B cells, and T cells. Single-cell transplantation assays have revealed that the HSC compartment contains lineage-restricted subtypes capable of reconstituting only specific lineages, such as platelets, alongside true multi-lineage HSCs. The proportion of these lineage-restricted HSCs increases with age, contributing to declined immunity and potentially to clonal haematopoiesis. To date, their identification and isolation have primarily relied on transgenic reporters, such as the Vwf-EGFP mouse line, which offer only moderate accuracy. In this study, we profiled highly purified adult mouse long-term HSCs (LT-HSCs; LSK CD48⁻ CD150⁺ CD34⁻) using the DOGMAseq platform, which enables simultaneous measurement of transcriptome (RNA-seq), chromatin accessibility (ATAC-seq), and surface protein expression (CITE-seq). We subsequently identified candidate clusters corresponding to platelet-biased and multi-lineage HSCs, and developed a fluorescence-activated cell sorting (FACS) strategy to enrich these populations. This strategy employs four surface markers detectable with commercially available antibodies and achieves higher enrichment specificity than the Vwf-EGFP reporter.","dates":{"publication":"2026/07/22"},"accession":"GSE328367","cross_references":{"GSM":["GSM9680307","GSM9680306","GSM9680305"],"GPL":["21103"],"GSE":["328367"],"taxon":["Mus musculus"],"PMID":["[42276065]"]}}