{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328630/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":[" Takifugu obscurus"," Periophthalmus magnuspinnatus","Gallus gallus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328630"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Shared and divergent transcriptional programs of oligodendrocyte differentiation across vertebrate species revealed by scRNA-seq analysis","description":"Myelination is essential for neural function in the vertebrate central nervous system, yet the molecular details of how the oligodendrocyte differentiation program has evolved remains poorly understood. Here, we performed a cross-species single-cell transcriptomic analysis of oligodendrocyte lineage cells in the spinal cord of five vertebrate species: fugu, mudskipper, chicken, mouse, and human. Pseudotime trajectory analysis revealed a shared OPC to COP to MOL differentiation trajectory across all species. Among stage-specific DEGs, 302 high-shared genes were organized into four co-expression modules, with 46 evolutionary core genes enriched for oligodendrocyte differentiation and Wnt signaling. Notably, 184 amniote-exclusive genes were enriched for synaptic vesicle transport and locomotory behavior, predominantly at the OPC stage, compared with only 18 teleost-exclusive genes, indicating that amniotes have expanded the oligodendrocyte differentiation program at the progenitor stage.","dates":{"publication":"2026/06/03"},"accession":"GSE328630","cross_references":{"GSM":["GSM9686224","GSM9686225","GSM9686226","GSM9686227","GSM9686228","GSM9686229"],"GPL":["24517","36844","36845"],"GSE":["328630"],"taxon":[" Takifugu obscurus"," Periophthalmus magnuspinnatus","Gallus gallus"],"PMID":["[42196265]"]}}