GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328636/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328636GEOGSEfalseRNA-seq Analysis of the 5-HT2B Receptor Agonist BW723C86 on OGD-induced HT22 Cells in a Transwell Co-culture System with BMDMsMicroglia and neurons engage in complex bidirectional communication that is crucial for the response to ischemic injury. To investigate the transcriptional changes underlying this crosstalk, we employed a Transwell co-culture system. Specifically, Bone marrow-derived macrophages（ BMDMs) were co-cultured with HT22 mouse hippocampal neurons subjected to 2 hours of oxygen-glucose deprivation (OGD) to model ischemic conditions. This setup allowed for the paracrine exchange of signals without direct cell contact. Following OGD, the co-culture was treated with either the 5-HT2B receptor agonist BW723C86 or a DMSO vehicle control. We then performed bulk RNA-sequencing on the microglial/BMDM population to profile their transcriptomic response to neuronal injury signals and receptor agonism. Our analysis aims to identify key signaling pathways and gene expression changes in microglia that are modulated by ischemic neurons and the serotonergic system, providing insights into the molecular mechanisms of neuroinflammation and neuroprotection.2026/07/03GSE328636GSM9686340GSM9686341GSM9686342GSM9686343GSM9686344GSM968634524247328636Mus musculus