GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328723/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328723GEOGSEfalseRNASeq dataset of human chondrocytes treated with caspase-1 and -8 inhibitors under osteoarthritis-like conditionsAbout 655 million persons worldwide are affected by osteoarthritis (OA), associated with excruciating chronic pain and disability in the elderly. Inflammation and cell death are two key events in OA and also key canonical functions of caspases. Since caspases play an important role in modulating inflammation, caspase inhibitors have been widely utilized to study diseases involving inflammation in animal models and clinical trials. Among these, key caspases, which are responsible for activation of the corresponding pathways include caspase-8 for the receptor mediated apoptotic pathway and caspase-1 as the key inflammatory caspase being involved in OA pathogenesis. OA- and non-OA chondrocytes were treated with caspase-1 and -8 inhibitors and TNFa. RNA was isolated after 3 days and processed for RNA sequencing. Data was pre-processed and analysed with R programming language v4.2.2. Downstream analysis included identification of differentially expressed genes providing a comprehensive overview of unique and shared transcriptional changes in both OA and non-OA cohorts. For technical validation RNA integrity was assessed and RNA-seq raw data were processed using Trimmomatic (SE mode) for adapter trimming and quality filtering.2026/08/25GSE328723GSM9688221GSM9688220GSM9688201GSM9688223GSM9688222GSM9688225GSM9688203GSM9688202GSM9688224GSM9688205GSM9688227GSM9688226GSM9688204GSM9688229GSM9688207GSM9688228GSM9688206GSM9688209GSM9688208GSM9688230GSM9688232GSM9688210GSM9688231GSM9688234GSM9688212GSM9688233GSM9688211GSM9688236GSM9688214GSM9688213GSM9688235GSM9688216GSM9688215GSM9688218GSM9688217GSM968821918573328723Homo sapiens