GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328878/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328878GEOGSEfalseTranscriptomic comparison of RA vs healthy monocytes undergoing different ex vivo activation conditionsRheumatoid arthritis (RA) monocytes are primed for inflammatory activation, but their disease-intrinsic molecular features have not been systematically profiled across omics layers. We aimed to define RA-associated alterations in primary human monocytes using an integrated multi-omics approach under controlled differentiation and stimulation conditions. Transcriptomics data showed upregulation of inflammatory and interferon-related programs but downregulation of translation- and mitochondrial matrix-associated gene sets in RA monocytes. Constraint-based metabolic modeling using transcript abundances as proxies for enzyme activities showed widespread downregulation of glycosylation-related metabolic fluxes.2026/04/28GSE328878GSM9693327GSM9693328GSM9693329GSM9693334GSM9693335GSM9693336GSM9693337GSM9693330GSM9693297GSM9693298GSM9693331GSM9693332GSM9693299GSM9693333GSM9693293GSM9693294GSM9693295GSM9693296GSM9693290GSM9693291GSM9693292GSM9693316GSM9693317GSM9693318GSM9693319GSM9693323GSM9693324GSM9693325GSM9693326GSM9693286GSM9693287GSM9693320GSM9693321GSM9693288GSM9693289GSM9693322GSM9693285GSM9693309GSM9693349GSM9693305GSM9693306GSM9693307GSM9693308GSM9693312GSM9693356GSM9693313GSM9693314GSM9693315GSM9693352GSM9693353GSM9693310GSM9693354GSM9693355GSM9693311GSM9693350GSM9693351GSM9693338GSM9693339GSM9693301GSM9693345GSM9693302GSM9693346GSM9693347GSM9693303GSM9693304GSM9693348GSM9693341GSM9693342GSM9693343GSM9693344GSM9693300GSM969334024676328878Homo sapiens